Quote:
==================================
Originally Posted by
yoderj@cox.net
I think the mold issue is coming from several sources:
1) I'm learning that how the nuts are collected and initially cared for is important. They are high in carbs and very susceptible to mold. Mold can transfer quickly from nut to nut. So, the source from which we get our nuts matters.
2) By storing nuts dry, depending on how dry, we may be slowing stratification. I previously posted the 10% & 65% moisture numbers but I don't even know how you would measure that. So, if stratification is slowed, the nuts will take longer to germinate once we do add moisture.
3) if there is any mold, and I've always had a few moldy nuts, it is a race between mold and germination. If the nuts are slower to germinate base on 2) above, then the mold has an advantage.
So, what do you do?.....
I think it depends on your constraints. If you are planning to fall plant and don't have to push to get your plants in the ground by early spring to get them established before summer heat, I think you do exactly what Big8 does. You simply add some damp sphagnum, keep them in the fridge to slow mold, and watch them daily. Keep in mind you will need some method to keep the orientation fixed. As soon as you see root radicle appearing, remove the nut and plant it in a rootmaker cell. This also holds true if you plant later in the spring and can water your trees when needed during the summer.
If you are in my situation, where you can't water during the summer and need the trees well established during the spring, I think you take your losses now like I did.
I removed my nuts from the fridge and put them in flats with long fiber sphagnum to germinate. I put the flats in bags to keep them moist. Out of 400 nuts I have had about 180 or so produce root radicles. I will probably get a few more before all is said an done, but I am now throwing many more nuts out due to mold each day than are germinating. I doubt I will get 200 which is a 50% rate.
However, I'm in a unique dilemma. I'm doing this in volume so I can't water my trees in the summer. They need to sink or swim on their own once planted. I would rather take my losses now at the nut stage than do all the work planting trees and loose the same 50% because the didn't get established before a summer drought.
So, if you can use the Big8 method, I would. If not, you may still get better results than me depending on how your nuts were cared for before you received them. Next year I plan to use Big8's nut source as well as his method of adding the damp peat a few weeks after cold storage.
Thanks,
Jack
==================================
Jack,
My trial of germination methods agrees with all you say above.
1) I used several different rinsing methods: 1/10 household bleach and water, 1/3 household hydrogen peroxide in water (1%), and plain, unfiltered tap water. There wasn't a big difference in time to germination with any of these. Those rinsed in tap water germinated a few days earlier but I doubt this is significant. The rinse solution doesn't seem to make a big difference in the rate of molding either.
2) I stored 20/55 of the nuts dry. None of these have germinated to date. I've lost none of these to mold. I'm going to put them all back in the frig in long-leaf, moistened peat and stratify them for another few weeks. We'll see if they make it.
3) The germination rate of the seeds stratified moist for 60 days was about 30%. I lost most of the remainder to mold. My experience is that 60 days in the cold isn't long enough.
Again, none of those stratified dry for 60 days have popped a root radicle. This tells me that moisture during the stratification process is necessary.
4) The germination rate for nuts stored moist for 90 days is about 80% so far.
5) Average time to germination has been about 6 days (range 4-14 days). I've lost 2/35 of those stratified for 90 days to mold. The remainder are still in the germination flat and looking pretty good (no mold).
6) About 1/2 of those that have developed top growth have the American characteristics (single bole) and the other half have multiple boles. Some of these are probably intermediate between the two but I don't know how to tell the difference.
Phil