Here is one of the relatively small number of studies that have investigated the seed size issue; however, it was conducted in Croatia.
https://www.mdpi.com/1999-4907/12/7/858
For those who do not wish to wander through the entire article ....
The Effect of Seed Size on Germination and Seedling Growth in Sweet Chestnut (Castanea sativa Mill.)
Institute of Forest Genetics, Dendrology and Botany, Faculty of Forestry and Wood Technology, University of Zagreb, HR-10000 Zagreb, Croatia
Abstract
"The quantity and quality of seedlings in the nursery has an impact on the success of re-establishment and later growth. High germination rates enable a sufficient number of seedlings, and their quality is assessed using a number of parameters, including seedling height and root collar diameter. These parameters are influenced in some species by seed size, but the correlation between them is species-specific. The model species in this research was sweet chestnut (Castanea sativa Mill.), and seeds from 12 populations from two distinct biogeographical regions of Croatia were collected. We examined the influence of seed size on four parameters: germination rate, seedling height, root collar diameter and sturdiness quotient. Seed size has been shown to have a positive influence on both seedling height and root collar diameter, whereas no such correlation was noted for germination rate and sturdiness quotient. Significant differences in nut size and seedling growth parameters were found between the Mediterranean and continental populations, with higher values observed in the coastal Mediterranean populations. We concluded that seed origin and seed size have a significant impact on seedling growth and are important factors to consider when choosing seed material. Further nursery operations should consider seed origin and local environmental conditions when choosing seedlings for reforestation efforts and general forest operations."
4. Conclusions and Practical Implications
"Differences in seed and seedling size can be attributed to intraspecific variability and the development of ecotypes. The Mediterranean populations in this research have been shown to have larger seeds, and their seedlings were characterized by more vigorous growth. These populations are genetically and environmentally specific and thus exhibit specific growth parameters. In addition, dry environmental conditions in their natural habitat could also influence the selection of larger seeds, as this provides increased drought tolerance."
"Seed size positively correlated with both seedling height and root collar diameter. In contrast, we found no evidence of the decisive influence of seed mass on germination rate and sturdiness quotient. Larger seeds give young seedlings a head start in early development by providing them with enough nutrients to spur growth. This aids early competition in the natural setting and increases the likelihood of this trait further surviving in the population. In contrast, the ideal conditions of stratification and later nursery treatments provided equal germination conditions for all seeds, regardless of their size. This could explain the lack of influence of seed mass on germination. By providing optimum and uniform germination conditions, nurseries can maximize the production of seedlings."
"Since chestnut seed and seedling quality is affected by seed origin and environment, choosing a proper seed source is an important factor in seedling production. Seed origin must be observed when selecting out-planting materials, especially when the conditions are unfavorable, e.g., drought stress. Properly selected seeds and seedlings ensure the success of reforestation and provide the best chance for the establishment of young plants. Choosing the right seed source for rootstock production is also of paramount importance in nursery operations, as it increases the viability of the grafted plant and provides a genetically more diverse rootstock, which is more likely to cope with the changing environment and possible new pests."